From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified protein | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
---|---|---|---|---|---|
Aprotinin | Bovine pancreatic powder | Magnetic chitosan particles | Trypsin | Elution with low pH buffer | [134] |
Concanavalin A | Jack bean extract | Magnetic particles | Dextran | Â | [68,135] |
Solanum tuberosum lectin | Potato tuber | Magnetic chitosan | Â | Elution with low pH buffer | [136] |
Green fluorescent protein (histidine tagged) | Â | Magnetic nanoparticles | Ni-NTA | Elution with imidazole containing buffer | [137] |
SIRT2 protein (recombinant, histidine tailed) | Human | Magnetic agarose beads | Ni-NTA | Elution with imidazole containing buffer | [139] |
Elongation factor (recombinant, histidine tailed) | Caenorhabditis elegans | Magnetic agarose beads | Ni-NTA | Elution with imidazole containing buffer | [140] |
Protein A | Recombinant Escherichia coli | Magnetic Eudragit | Human IgG | Magnetic two-phase system | [141] |
Tumor necrosis factor (TNF) | Â | Dynabeads M-280 | Mouse monoclonal antibody | Solid phase immunoassay | [142] |
Anti-MUC1 diabody fragment | Recombinant Escherichia coli | Magnetic agarose beads | Specific peptide | Â | [143] |
MHC class II molecules | MDCK cells | Dynabeads M-450 rat anti-mouse IgG1 | Specific antibodies | Elution with SDS-PAGE buffer | [144] |
Lamin B3 | Xenopus egg extracts | Dynabeads | Specific antibodies | Elution with 6 M urea | [44] |
6x-His-tagged proteins | Human fibroblasts | Magnetic agarose beads | Ni-NTA | Elution with imidazole containing buffer | [145] |
Estrogen receptor | Adipose tissue | Dynabeads M-280 streptavidin | Biotinylated monoclonal mouse anti-human estrogen receptor antibody | Indirect method | [146] |
Thiol-reactive chromatin restriction fragments | Mouse fibroblasts | Mercurated agarose magnetic beads | p-Hydroxymercuribenzoate | Elution with 0.5 M NaCl and 20 mM dithiothreitol | [147] |
L1 coat protein | Human papillomavirus | Magnetic polyglutaraldehyde particles | Iminodiacetic acid charged with Cu2+ | Elution with imidazole containing buffer | [41] |
Insulin receptor | Rat muscle or liver extract | Dynabeads M-450 | Anti-P5 antibody | SDS PAGE analysis | [148] |
Stat3 | DER cells | Dynabeads | Biotinylated tyrosine phosphorylated peptides | SDS PAGE analysis | [149] |
Transferrin receptor | Human | Dynabeads M-450 sheep anti-mouse IgG1 | Anti-human transferrin receptor monoclonal antibody | SDS analysis | [151] |
Prion protein PrPSc | Brain extract | Dynabeads M-280 tosyl activated | Plasminogen | SDS analysis | [39,40] |
Biotinylated proteins from extracellular matrix | Bipolaris sorokiniana | Dynabeads | Streptavidin | SDS analysis | [152] |
Cryoprotectin | Leaves of cold-acclimated cabbage (Brassica oleracea) | Dynabeads-protein A | Specific antibody | Â | [153] |
Prostate specific antigen | Serum from a prostate cancer suffering patient | Streptavidin-coated magnetic beads | Biotinylated monoclonal antibody | Elution with low pH solution | [154,155] |
Estrogen receptor | In vitro translation | Magnetic beads | Antibody | Elution with SDS buffer | [156] |
VHDL receptor | Helicoverpa zea | Streptavidin-coated magnetic beads | VHDL-biotin ligand | Â | [157] |
Fructosyllysine-specific binding protein | U937 cells | Dynabeads M-280 tosylactivated | Poly-L-lysine-glucose conjugate | Two proteins isolated | [158] |
Ubiquitin (histidine tagged) | Â | Nickel-gold nanorods | Â | Elution with acidic buffer | [18] |