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Table 8 Examples of other proteins purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified protein

Source

Magnetic carrier

Affinity ligand

Further details

Reference

Aprotinin

Bovine pancreatic powder

Magnetic chitosan particles

Trypsin

Elution with low pH buffer

[134]

Concanavalin A

Jack bean extract

Magnetic particles

Dextran

 

[68,135]

Solanum tuberosum lectin

Potato tuber

Magnetic chitosan

 

Elution with low pH buffer

[136]

Green fluorescent protein (histidine tagged)

 

Magnetic nanoparticles

Ni-NTA

Elution with imidazole containing buffer

[137]

SIRT2 protein (recombinant, histidine tailed)

Human

Magnetic agarose beads

Ni-NTA

Elution with imidazole containing buffer

[139]

Elongation factor (recombinant, histidine tailed)

Caenorhabditis elegans

Magnetic agarose beads

Ni-NTA

Elution with imidazole containing buffer

[140]

Protein A

Recombinant Escherichia coli

Magnetic Eudragit

Human IgG

Magnetic two-phase system

[141]

Tumor necrosis factor (TNF)

 

Dynabeads M-280

Mouse monoclonal antibody

Solid phase immunoassay

[142]

Anti-MUC1 diabody fragment

Recombinant Escherichia coli

Magnetic agarose beads

Specific peptide

 

[143]

MHC class II molecules

MDCK cells

Dynabeads M-450 rat anti-mouse IgG1

Specific antibodies

Elution with SDS-PAGE buffer

[144]

Lamin B3

Xenopus egg extracts

Dynabeads

Specific antibodies

Elution with 6 M urea

[44]

6x-His-tagged proteins

Human fibroblasts

Magnetic agarose beads

Ni-NTA

Elution with imidazole containing buffer

[145]

Estrogen receptor

Adipose tissue

Dynabeads M-280 streptavidin

Biotinylated monoclonal mouse anti-human estrogen receptor antibody

Indirect method

[146]

Thiol-reactive chromatin restriction fragments

Mouse fibroblasts

Mercurated agarose magnetic beads

p-Hydroxymercuribenzoate

Elution with 0.5 M NaCl and 20 mM dithiothreitol

[147]

L1 coat protein

Human papillomavirus

Magnetic polyglutaraldehyde particles

Iminodiacetic acid charged with Cu2+

Elution with imidazole containing buffer

[41]

Insulin receptor

Rat muscle or liver extract

Dynabeads M-450

Anti-P5 antibody

SDS PAGE analysis

[148]

Stat3

DER cells

Dynabeads

Biotinylated tyrosine phosphorylated peptides

SDS PAGE analysis

[149]

Transferrin receptor

Human

Dynabeads M-450 sheep anti-mouse IgG1

Anti-human transferrin receptor monoclonal antibody

SDS analysis

[151]

Prion protein PrPSc

Brain extract

Dynabeads M-280 tosyl activated

Plasminogen

SDS analysis

[39,40]

Biotinylated proteins from extracellular matrix

Bipolaris sorokiniana

Dynabeads

Streptavidin

SDS analysis

[152]

Cryoprotectin

Leaves of cold-acclimated cabbage (Brassica oleracea)

Dynabeads-protein A

Specific antibody

 

[153]

Prostate specific antigen

Serum from a prostate cancer suffering patient

Streptavidin-coated magnetic beads

Biotinylated monoclonal antibody

Elution with low pH solution

[154,155]

Estrogen receptor

In vitro translation

Magnetic beads

Antibody

Elution with SDS buffer

[156]

VHDL receptor

Helicoverpa zea

Streptavidin-coated magnetic beads

VHDL-biotin ligand

 

[157]

Fructosyllysine-specific binding protein

U937 cells

Dynabeads M-280 tosylactivated

Poly-L-lysine-glucose conjugate

Two proteins isolated

[158]

Ubiquitin (histidine tagged)

 

Nickel-gold nanorods

 

Elution with acidic buffer

[18]