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Table 8 Examples of other proteins purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified protein Source Magnetic carrier Affinity ligand Further details Reference
Aprotinin Bovine pancreatic powder Magnetic chitosan particles Trypsin Elution with low pH buffer [134]
Concanavalin A Jack bean extract Magnetic particles Dextran   [68,135]
Solanum tuberosum lectin Potato tuber Magnetic chitosan   Elution with low pH buffer [136]
Green fluorescent protein (histidine tagged)   Magnetic nanoparticles Ni-NTA Elution with imidazole containing buffer [137]
SIRT2 protein (recombinant, histidine tailed) Human Magnetic agarose beads Ni-NTA Elution with imidazole containing buffer [139]
Elongation factor (recombinant, histidine tailed) Caenorhabditis elegans Magnetic agarose beads Ni-NTA Elution with imidazole containing buffer [140]
Protein A Recombinant Escherichia coli Magnetic Eudragit Human IgG Magnetic two-phase system [141]
Tumor necrosis factor (TNF)   Dynabeads M-280 Mouse monoclonal antibody Solid phase immunoassay [142]
Anti-MUC1 diabody fragment Recombinant Escherichia coli Magnetic agarose beads Specific peptide   [143]
MHC class II molecules MDCK cells Dynabeads M-450 rat anti-mouse IgG1 Specific antibodies Elution with SDS-PAGE buffer [144]
Lamin B3 Xenopus egg extracts Dynabeads Specific antibodies Elution with 6 M urea [44]
6x-His-tagged proteins Human fibroblasts Magnetic agarose beads Ni-NTA Elution with imidazole containing buffer [145]
Estrogen receptor Adipose tissue Dynabeads M-280 streptavidin Biotinylated monoclonal mouse anti-human estrogen receptor antibody Indirect method [146]
Thiol-reactive chromatin restriction fragments Mouse fibroblasts Mercurated agarose magnetic beads p-Hydroxymercuribenzoate Elution with 0.5 M NaCl and 20 mM dithiothreitol [147]
L1 coat protein Human papillomavirus Magnetic polyglutaraldehyde particles Iminodiacetic acid charged with Cu2+ Elution with imidazole containing buffer [41]
Insulin receptor Rat muscle or liver extract Dynabeads M-450 Anti-P5 antibody SDS PAGE analysis [148]
Stat3 DER cells Dynabeads Biotinylated tyrosine phosphorylated peptides SDS PAGE analysis [149]
Transferrin receptor Human Dynabeads M-450 sheep anti-mouse IgG1 Anti-human transferrin receptor monoclonal antibody SDS analysis [151]
Prion protein PrPSc Brain extract Dynabeads M-280 tosyl activated Plasminogen SDS analysis [39,40]
Biotinylated proteins from extracellular matrix Bipolaris sorokiniana Dynabeads Streptavidin SDS analysis [152]
Cryoprotectin Leaves of cold-acclimated cabbage (Brassica oleracea) Dynabeads-protein A Specific antibody   [153]
Prostate specific antigen Serum from a prostate cancer suffering patient Streptavidin-coated magnetic beads Biotinylated monoclonal antibody Elution with low pH solution [154,155]
Estrogen receptor In vitro translation Magnetic beads Antibody Elution with SDS buffer [156]
VHDL receptor Helicoverpa zea Streptavidin-coated magnetic beads VHDL-biotin ligand   [157]
Fructosyllysine-specific binding protein U937 cells Dynabeads M-280 tosylactivated Poly-L-lysine-glucose conjugate Two proteins isolated [158]
Ubiquitin (histidine tagged)   Nickel-gold nanorods   Elution with acidic buffer [18]