From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified protein | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
---|---|---|---|---|---|
Albumin, bovine serum | Commercial preparation | Magnetic agar beads | Cibacron blue3GA | Adsorption experiments | [126] |
 | Commercial preparation | Magnetic cross-linked polyvinylalcohol | Cibacron blue3GA | Adsorption experiments | [76,127] |
 |  | Magnetic chitosan microspheres | Cibacron blue3GA |  | [78] |
 | Commercial preparation | Magnetic poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) particles |  | Anion exchange separation | [128] |
 | Commercial preparation | Magnetic poly(ethylene glycol dimethacrylate-co-N-methacryloyl-(L)-histidine methyl ester) microbeads | Cu2+ | Elution with 1.0 M NaSCN | [129] |
Albumin, human serum | Commercial preparation | Magnetic poly(2-hydroxyethylmethacrylate) beads | Iminodiacetic acid charged with Cu2+ | Elution with 1.0 M NaSCN | [130] |
 | Human plasma | Magnetic poly(2-hydroxyethyl methacrylate) beads | Cibacron blue F3GA | Elution with 0.5 M NaSCN | [131] |
 | Commercial preparation | Magnetic particles covered with thermosensitive polymer | - | Desorption by decreasing temperature | [132,133] |
Albumin, human serum (recombinant, FLAG tagged) | Yeast cells | Magnetic glass beads | Anti-FLAG antibody | Elution with EDTA containing buffer | [1] |
Glycated haemoglobin | Human blood | Magnetic poly(vinyl alcohol) beads | m-Aminophenyl-boronic acid | Elution with sorbitol | [138] |
Haemoglobin | Bovine, commercial preparation | Amine terminated iron oxide particles | Iminodiacetic acid charged with Cu2+ | Elution with imidazole containing buffer | [63] |
Haemoglobin A1c | Human blood | Magnetic particles isolated from Magnetospirillum magneticum AMB-1 | m-Aminophenyl-boronic acid | used for affinity immunoassay | [150] |