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Table 7 Purification of albumin and haemoglobin by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified protein Source Magnetic carrier Affinity ligand Further details Reference
Albumin, bovine serum Commercial preparation Magnetic agar beads Cibacron blue3GA Adsorption experiments [126]
  Commercial preparation Magnetic cross-linked polyvinylalcohol Cibacron blue3GA Adsorption experiments [76,127]
   Magnetic chitosan microspheres Cibacron blue3GA   [78]
  Commercial preparation Magnetic poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) particles   Anion exchange separation [128]
  Commercial preparation Magnetic poly(ethylene glycol dimethacrylate-co-N-methacryloyl-(L)-histidine methyl ester) microbeads Cu2+ Elution with 1.0 M NaSCN [129]
Albumin, human serum Commercial preparation Magnetic poly(2-hydroxyethylmethacrylate) beads Iminodiacetic acid charged with Cu2+ Elution with 1.0 M NaSCN [130]
  Human plasma Magnetic poly(2-hydroxyethyl methacrylate) beads Cibacron blue F3GA Elution with 0.5 M NaSCN [131]
  Commercial preparation Magnetic particles covered with thermosensitive polymer - Desorption by decreasing temperature [132,133]
Albumin, human serum (recombinant, FLAG tagged) Yeast cells Magnetic glass beads Anti-FLAG antibody Elution with EDTA containing buffer [1]
Glycated haemoglobin Human blood Magnetic poly(vinyl alcohol) beads m-Aminophenyl-boronic acid Elution with sorbitol [138]
Haemoglobin Bovine, commercial preparation Amine terminated iron oxide particles Iminodiacetic acid charged with Cu2+ Elution with imidazole containing buffer [63]
Haemoglobin A1c Human blood Magnetic particles isolated from Magnetospirillum magneticum AMB-1 m-Aminophenyl-boronic acid used for affinity immunoassay [150]