From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified protein | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
---|---|---|---|---|---|
CUG binding proteins | Human myoblasts or fibroblasts | Dynabeads M-280 streptavidin | Biotinylated(CUG)10 | Elution with 1 M NaCl | [103] |
Transcription factor Ï„ | Saccharomyces cerevisiae | Dynabeads M-280 streptavidin | Biotinylated tRNAGlu gene fragment | Elution with high salt buffer | [104,105] |
DNA-binding proteins | Crude tissue extract | Magnetic phospho cellulose particles | Â | Â | [106] |
DNA-binding proteins | Escherichia coli | Magnetic phospho cellulose particles | Â | Â | [107] |
DNA-binding proteins | HeLa nuclear extracts | Dynabeads M-280 streptavidin | Biotin-labelled DNA fragment | Elution with 2 M NaCl | [108] |
Vaccinia virus early transcription factor | Vaccinia virions | Dynabeads M-280 streptavidin | Biotinylated double-stranded DNA | Elution with high salt buffer | [109] |
Ecdysteroid receptor | Drosophila melanogaster nuclear extract | Dynabeads M-280 streptavidin | Biotinylated double-stranded oligonucleotide | Elution with 0.4 M KCl | [110] |
NanR protein (recombinant) | Escherichia coli | μMACS streptavidin MicroBeads | Biotin-labelled DNA fragment | Elution with 1 M NaCl | [111] |
p27 | Rabbit hepatocytes | Dynabeads M-280 streptavidin | Guanine-rich single-stranded DNA | Elution with NaCl solution | [112] |
Pigpen protein | Endothelial cells | Magnetic streptavidin beads | Biotinylated aptamer | Elution with 1 M NaCl | [113] |
RNA binding proteins | Saccharomyces cerevisiae | μMACS streptavidin MicroBeads | Biotin-labelled RNA probe | Elution with 1 M NaCl | [114] |
Single-stranded telomere binding protein (sTBP) | Nuclei from vertebrate tissues | Dynabeads M-280 streptavidin | Biotinylated single stranded TTAGGGn repeats | Elution with high salt buffer | [115] |
Transcription proteins | Human myeloid cells | Dynabeads M-280 streptavidin | Biotinylated serum inducible element (hSIE) | Elution with high salt buffer | [116] |
Transcription factor γRF-1 | Human monocytes and epidermal cells | Dynabeads M-280 streptavidin | Biotinylated DNA containing γRF-1 sequences | Elution with 0.6 M KCl | [117] |
Protein factor MS2 | Murine skeletal myotubes | Dynabeads | Double-stranded DNA | Elution with 100 mM sodium acetate, pH 4.2 | [118] |
Guide RNA binding protein | Trypanosoma brucei mitochondria | Dynabeads M-450 goat anti-mouse IgG | Monoclonal antibody | Elution with low pH buffer cont. SDS | [119] |
RNA binding proteins | Pollen grains | Streptavidin MagneSphere particles | Biotinylated oligonucleotides | Elution with SDS buffer | [120] |
DNA binding protein | Schistosoma mansoni | Dynabeads M-280 streptavidin | Biotinylated DNA | Elution with sodium acetate buffer | [121] |
ssDNA binding proteins | Transfected mouse fibroblasts | Dynabeads anti-rabbit IgG | Rabbit antibody | Indirect method | [122] |
Tenascin-C | Glioblastoma cells | Dynabeads streptavidin | Biotinylated aptamer | Elution with high salt buffer | [123] |
Thermostable brain factor (ThBF) | Rat brain | Streptavidin magnetic particles | Biotinylated oligonucleotides | Elution with 0.7 M KCl | [124] |
TTF1 protein | Escherichia coli lysate | Dynabeads M-280 streptavidin | Biotinylated aptamer | Elution with DNase | [125] |