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Table 6 Examples of DNA/RNA/oligonucleotide/aptamer binding proteins purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified protein Source Magnetic carrier Affinity ligand Further details Reference
CUG binding proteins Human myoblasts or fibroblasts Dynabeads M-280 streptavidin Biotinylated(CUG)10 Elution with 1 M NaCl [103]
Transcription factor τ Saccharomyces cerevisiae Dynabeads M-280 streptavidin Biotinylated tRNAGlu gene fragment Elution with high salt buffer [104,105]
DNA-binding proteins Crude tissue extract Magnetic phospho cellulose particles    [106]
DNA-binding proteins Escherichia coli Magnetic phospho cellulose particles    [107]
DNA-binding proteins HeLa nuclear extracts Dynabeads M-280 streptavidin Biotin-labelled DNA fragment Elution with 2 M NaCl [108]
Vaccinia virus early transcription factor Vaccinia virions Dynabeads M-280 streptavidin Biotinylated double-stranded DNA Elution with high salt buffer [109]
Ecdysteroid receptor Drosophila melanogaster nuclear extract Dynabeads M-280 streptavidin Biotinylated double-stranded oligonucleotide Elution with 0.4 M KCl [110]
NanR protein (recombinant) Escherichia coli μMACS streptavidin MicroBeads Biotin-labelled DNA fragment Elution with 1 M NaCl [111]
p27 Rabbit hepatocytes Dynabeads M-280 streptavidin Guanine-rich single-stranded DNA Elution with NaCl solution [112]
Pigpen protein Endothelial cells Magnetic streptavidin beads Biotinylated aptamer Elution with 1 M NaCl [113]
RNA binding proteins Saccharomyces cerevisiae μMACS streptavidin MicroBeads Biotin-labelled RNA probe Elution with 1 M NaCl [114]
Single-stranded telomere binding protein (sTBP) Nuclei from vertebrate tissues Dynabeads M-280 streptavidin Biotinylated single stranded TTAGGGn repeats Elution with high salt buffer [115]
Transcription proteins Human myeloid cells Dynabeads M-280 streptavidin Biotinylated serum inducible element (hSIE) Elution with high salt buffer [116]
Transcription factor γRF-1 Human monocytes and epidermal cells Dynabeads M-280 streptavidin Biotinylated DNA containing γRF-1 sequences Elution with 0.6 M KCl [117]
Protein factor MS2 Murine skeletal myotubes Dynabeads Double-stranded DNA Elution with 100 mM sodium acetate, pH 4.2 [118]
Guide RNA binding protein Trypanosoma brucei mitochondria Dynabeads M-450 goat anti-mouse IgG Monoclonal antibody Elution with low pH buffer cont. SDS [119]
RNA binding proteins Pollen grains Streptavidin MagneSphere particles Biotinylated oligonucleotides Elution with SDS buffer [120]
DNA binding protein Schistosoma mansoni Dynabeads M-280 streptavidin Biotinylated DNA Elution with sodium acetate buffer [121]
ssDNA binding proteins Transfected mouse fibroblasts Dynabeads anti-rabbit IgG Rabbit antibody Indirect method [122]
Tenascin-C Glioblastoma cells Dynabeads streptavidin Biotinylated aptamer Elution with high salt buffer [123]
Thermostable brain factor (ThBF) Rat brain Streptavidin magnetic particles Biotinylated oligonucleotides Elution with 0.7 M KCl [124]
TTF1 protein Escherichia coli lysate Dynabeads M-280 streptavidin Biotinylated aptamer Elution with DNase [125]