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Table 6 Examples of DNA/RNA/oligonucleotide/aptamer binding proteins purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified protein

Source

Magnetic carrier

Affinity ligand

Further details

Reference

CUG binding proteins

Human myoblasts or fibroblasts

Dynabeads M-280 streptavidin

Biotinylated(CUG)10

Elution with 1 M NaCl

[103]

Transcription factor Ï„

Saccharomyces cerevisiae

Dynabeads M-280 streptavidin

Biotinylated tRNAGlu gene fragment

Elution with high salt buffer

[104,105]

DNA-binding proteins

Crude tissue extract

Magnetic phospho cellulose particles

  

[106]

DNA-binding proteins

Escherichia coli

Magnetic phospho cellulose particles

  

[107]

DNA-binding proteins

HeLa nuclear extracts

Dynabeads M-280 streptavidin

Biotin-labelled DNA fragment

Elution with 2 M NaCl

[108]

Vaccinia virus early transcription factor

Vaccinia virions

Dynabeads M-280 streptavidin

Biotinylated double-stranded DNA

Elution with high salt buffer

[109]

Ecdysteroid receptor

Drosophila melanogaster nuclear extract

Dynabeads M-280 streptavidin

Biotinylated double-stranded oligonucleotide

Elution with 0.4 M KCl

[110]

NanR protein (recombinant)

Escherichia coli

μMACS streptavidin MicroBeads

Biotin-labelled DNA fragment

Elution with 1 M NaCl

[111]

p27

Rabbit hepatocytes

Dynabeads M-280 streptavidin

Guanine-rich single-stranded DNA

Elution with NaCl solution

[112]

Pigpen protein

Endothelial cells

Magnetic streptavidin beads

Biotinylated aptamer

Elution with 1 M NaCl

[113]

RNA binding proteins

Saccharomyces cerevisiae

μMACS streptavidin MicroBeads

Biotin-labelled RNA probe

Elution with 1 M NaCl

[114]

Single-stranded telomere binding protein (sTBP)

Nuclei from vertebrate tissues

Dynabeads M-280 streptavidin

Biotinylated single stranded TTAGGGn repeats

Elution with high salt buffer

[115]

Transcription proteins

Human myeloid cells

Dynabeads M-280 streptavidin

Biotinylated serum inducible element (hSIE)

Elution with high salt buffer

[116]

Transcription factor γRF-1

Human monocytes and epidermal cells

Dynabeads M-280 streptavidin

Biotinylated DNA containing γRF-1 sequences

Elution with 0.6 M KCl

[117]

Protein factor MS2

Murine skeletal myotubes

Dynabeads

Double-stranded DNA

Elution with 100 mM sodium acetate, pH 4.2

[118]

Guide RNA binding protein

Trypanosoma brucei mitochondria

Dynabeads M-450 goat anti-mouse IgG

Monoclonal antibody

Elution with low pH buffer cont. SDS

[119]

RNA binding proteins

Pollen grains

Streptavidin MagneSphere particles

Biotinylated oligonucleotides

Elution with SDS buffer

[120]

DNA binding protein

Schistosoma mansoni

Dynabeads M-280 streptavidin

Biotinylated DNA

Elution with sodium acetate buffer

[121]

ssDNA binding proteins

Transfected mouse fibroblasts

Dynabeads anti-rabbit IgG

Rabbit antibody

Indirect method

[122]

Tenascin-C

Glioblastoma cells

Dynabeads streptavidin

Biotinylated aptamer

Elution with high salt buffer

[123]

Thermostable brain factor (ThBF)

Rat brain

Streptavidin magnetic particles

Biotinylated oligonucleotides

Elution with 0.7 M KCl

[124]

TTF1 protein

Escherichia coli lysate

Dynabeads M-280 streptavidin

Biotinylated aptamer

Elution with DNase

[125]