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Table 5 Examples of antibodies purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified antibody Source Magnetic carrier Affinity ligand Further details Reference
Anti-BODIPY-fluorescein antibodies   Magnetoliposomes BODIPY-fluorescein   [94]
Anti-DNA antibody Systemic lupus erythematosus patient plasma Magnetic poly(2-hydroxyethyl-methacrylate) beads DNA Desorption with 1 M NaSCN solution [95]
Anti-human chorionic gonadotropin antibody Murine ascites supernatants Magnetic cellulose beads Human chorionic gonadotropin   [96]
Antibody (from rat) Sample from affinity chromatography Dynabeads M-280 Sheep anti-rabbit IgG Antibody concentration [3]
Antibody Rabbit serum Dynabeads M-280 Sheep anti-rabbit IgG Elution with 0.5 M acetic acid [97]
Monoclonal antibodies Mouse hybridoma culture broth Magnetite particles Protein A   [98]
Anti-bovine serum albumin antibodies   Thermosensitive magnetic microspheres Bovine serum albumin Immobilization by the carbodiimide method [99]
Immunoglobulin G, human Commercial preparation Magnetic poly(ethylene glycol dimethacrylate-N-methacryloly-L-histidine-methylester) beads   Elution with 1 M NaCl [100]
Immunoglobulin G Blood serum Carboxyl-terminated magnetic particles MproteinAG   [101]
IgE antibodies Allergic patients sera Magnetoliposomes Antigenic proteins   [16]
Murine anti-fibroblast growth factor receptor 1 IgM Ascites Polystyrene magnetic beads Rat anti-mouse IgM monoclonal antibody   [102]