Magnetic techniques for the isolation and purification of proteins and peptides

Safarik, Ivo; Safarikova, Mirka

doi:10.1186/1477-044X-2-7

BioMagnetic Research and Technology

Table 4 Examples of other enzymes purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme	Source	Magnetic carrier	Affinity ligand	Further details	Reference
Alcohol dehydrogenase	Yeast homogenate	Magnetic cross-linked polyvinylalcohol	Cibacron blue 3GA	Elution with high salt buffer	[52]
	Saccharomyces cerevisiae extract		PEG with bound Cibacron blue	Magnetic two-phase system	[53]
Aldolase (recombinant, histidine tagged)	Pea	Magnetic core and nickel-silica composite matrix	Ni²⁺	Elution with imidazole containing buffer	[36]
AngioI-TEM-β-lactamase	Escherichia coli cells extracts	Magnetic agarose beads	Iminodiacetic acid charged with Zn²⁺	Elution with low pH buffer	[56]
Asparaginase	Escherichia coli homogenate	Magnetic polyacrylamide gel particles	D-Asparagine	Elution with D-asparagine solution	[58]
Carbonic anhydrase	Model mixture	Magnetic agarose beads	Sulfanilimide	Elution with high salt buffer	[14]
Catalase	Bovine liver, commercial preparation	Magnetic poly(EGDMA-MAH) beads	Fe³⁺	Elution with NaSCN solution	[61]
Cytochrome c	Horse, Candida krusei	Amine terminated iron oxide particles	Iminodiacetic acid charged with Cu²⁺	Binding studies	[63]
	Commercial preparation	Au@magnetic particles		MALDI MS analysis	[31]
	Horse heart	Magnetic agarose beads	Iminodiacetic acid charged with Cu²⁺	Elution with EDTA containing buffer	[56]
	Bovine heart	Magnetic ion-exchange particles		Protein binding studies	[12]
Glucose-6-phosphate dehydrogenase		Ferrofluid modified Sepharose 4B	ADP		[65]
	Saccharomyces cerevisiae extract		PEG with bound Cibacron blue	Magnetic two-phase system	[53]
Hexokinase	Escherichia coli homogenate		PEG with bound Cibacron blue	Magnetic two-phase system	[53]
Lactate dehydrogenase	Beef heart	Ferrofluid modified Sepharose 4B	AMP	Elution with 1 mM NADH	[13]
	Porcine muscle	Magnetic agarose beads	Reactive Red 120	Column elution with NaCl gradient	[66]
Lactoperoxidase	Sweet whey	Magnetic cation exchanger		HGMS	[67,68]
Luciferase (histidine-tagged)	Escherichia coli homogenate	MagneHis™ system	Ni²⁺		[69,70]
Phosphatase, alkaline	Human placenta	Dynabeads M-450	Specific antibody	Activity of bound enzyme measured	[83]
Phosphatase, alkaline (fusion protein comprising the DNA-binding lac repressor)	Bacterial lysate	Magnetic beads	DNA containing Escherichia coli lac operator	Elution with lactose analogue	[64]
Phosphofructokinase	Saccharomyces cerevisiae extract		PEG with bound Cibacron blue	Magnetic two-phase system	[53]
6-Phosphogluconate dehydrogenase		Ferrofluid modified Sepharose 4B	ADP	Elution with 1 mM NADP	[13]
Thioredoxin (recombinant, histidine-tagged)	Escherichia coli	Magnetic agarose	Ni-NTA	Elution with imidazole containing buffer	[20]
tRNA methionyl synthetase (recombinant, histidine-tagged)	Escherichia coli	MagneHis™ system	Ni²⁺	Rapid detection and quantitation of isolated protein	[85]
Uricase (recombinant, histidine-tailed)	Bacillus	Ion-chelating magnetic agarose beads	Ni²⁺	Elution by cleavage with proteinase K	[92]

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