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Table 4 Examples of other enzymes purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme Source Magnetic carrier Affinity ligand Further details Reference
Alcohol dehydrogenase Yeast homogenate Magnetic cross-linked polyvinylalcohol Cibacron blue 3GA Elution with high salt buffer [52]
  Saccharomyces cerevisiae extract   PEG with bound Cibacron blue Magnetic two-phase system [53]
Aldolase (recombinant, histidine tagged) Pea Magnetic core and nickel-silica composite matrix Ni2+ Elution with imidazole containing buffer [36]
AngioI-TEM-β-lactamase Escherichia coli cells extracts Magnetic agarose beads Iminodiacetic acid charged with Zn2+ Elution with low pH buffer [56]
Asparaginase Escherichia coli homogenate Magnetic polyacrylamide gel particles D-Asparagine Elution with D-asparagine solution [58]
Carbonic anhydrase Model mixture Magnetic agarose beads Sulfanilimide Elution with high salt buffer [14]
Catalase Bovine liver, commercial preparation Magnetic poly(EGDMA-MAH) beads Fe3+ Elution with NaSCN solution [61]
Cytochrome c Horse, Candida krusei Amine terminated iron oxide particles Iminodiacetic acid charged with Cu2+ Binding studies [63]
  Commercial preparation Au@magnetic particles   MALDI MS analysis [31]
  Horse heart Magnetic agarose beads Iminodiacetic acid charged with Cu2+ Elution with EDTA containing buffer [56]
  Bovine heart Magnetic ion-exchange particles   Protein binding studies [12]
Glucose-6-phosphate dehydrogenase   Ferrofluid modified Sepharose 4B ADP   [65]
  Saccharomyces cerevisiae extract   PEG with bound Cibacron blue Magnetic two-phase system [53]
Hexokinase Escherichia coli homogenate   PEG with bound Cibacron blue Magnetic two-phase system [53]
Lactate dehydrogenase Beef heart Ferrofluid modified Sepharose 4B AMP Elution with 1 mM NADH [13]
  Porcine muscle Magnetic agarose beads Reactive Red 120 Column elution with NaCl gradient [66]
Lactoperoxidase Sweet whey Magnetic cation exchanger   HGMS [67,68]
Luciferase (histidine-tagged) Escherichia coli homogenate MagneHis™ system Ni2+   [69,70]
Phosphatase, alkaline Human placenta Dynabeads M-450 Specific antibody Activity of bound enzyme measured [83]
Phosphatase, alkaline (fusion protein comprising the DNA-binding lac repressor) Bacterial lysate Magnetic beads DNA containing Escherichia coli lac operator Elution with lactose analogue [64]
Phosphofructokinase Saccharomyces cerevisiae extract   PEG with bound Cibacron blue Magnetic two-phase system [53]
6-Phosphogluconate dehydrogenase   Ferrofluid modified Sepharose 4B ADP Elution with 1 mM NADP [13]
Thioredoxin (recombinant, histidine-tagged) Escherichia coli Magnetic agarose Ni-NTA Elution with imidazole containing buffer [20]
tRNA methionyl synthetase (recombinant, histidine-tagged) Escherichia coli MagneHis™ system Ni2+ Rapid detection and quantitation of isolated protein [85]
Uricase (recombinant, histidine-tailed) Bacillus Ion-chelating magnetic agarose beads Ni2+ Elution by cleavage with proteinase K [92]