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Table 3 Examples of polysaccharide and disaccharide hydrolases purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme Source Magnetic carrier Affinity ligand Further details Reference
α-Amylases Porcine pancreas, Bacillus subtilis, wheat germ Magnetic alginate beads   Elution with 1 M maltose [4]
  Bacillus amyloliquefaciens, porcine pancreas Magnetic alginate microbeads   Elution with 1 M maltose [10]
β-Amylase Sweet potato Magnetic alginate beads   Elution with 1 M maltose [55]
β-Galactosidase Escherichia coli homogenate Silanized magnetite p-Aminophenyl-β-D-thiogalactopyranoside Elution with borate buffer, pH 10 [58]
β-Galactosidase (fusion protein comprising the DNA-binding lac repressor) Bacterial lysate Magnetic beads DNA containing Escherichia coli lac operator Elution with lactose analogue [64]
Glucoamylase Aspergillus niger Magnetic alginate beads   Elution with 1 M maltose [55]
Pectinase Commercial preparation Magnetic alginate beads    [82]
Pullulanase Bacillus acidopullulyticus Magnetic alginate beads   Elution with 1 M maltose [55]