Magnetic techniques for the isolation and purification of proteins and peptides

Table 3 Examples of polysaccharide and disaccharide hydrolases purified by magnetic techniques

Purified enzyme	Source	Magnetic carrier	Affinity ligand	Further details	Reference
α-Amylases	Porcine pancreas, Bacillus subtilis, wheat germ	Magnetic alginate beads		Elution with 1 M maltose	[4]
	Bacillus amyloliquefaciens, porcine pancreas	Magnetic alginate microbeads		Elution with 1 M maltose	[10]
β-Amylase	Sweet potato	Magnetic alginate beads		Elution with 1 M maltose	[55]
β-Galactosidase	Escherichia coli homogenate	Silanized magnetite	p-Aminophenyl-β-D-thiogalactopyranoside	Elution with borate buffer, pH 10	[58]
β-Galactosidase (fusion protein comprising the DNA-binding lac repressor)	Bacterial lysate	Magnetic beads	DNA containing Escherichia coli lac operator	Elution with lactose analogue	[64]
Glucoamylase	Aspergillus niger	Magnetic alginate beads		Elution with 1 M maltose	[55]
Pectinase	Commercial preparation	Magnetic alginate beads			[82]
Pullulanase	Bacillus acidopullulyticus	Magnetic alginate beads		Elution with 1 M maltose	[55]