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Table 2 Purification of lysozyme by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme Source Magnetic carrier Affinity ligand Further details Reference
Lysozyme Hen egg white Magnetic chitin   Elution with 0.01 M HCl [71]
  Hen egg white Magnetic acetylated chitosan   Elution with 0.01 M HCl [9]
  Commercial preparation Magnetic poly(2-hydroxyethyl methacrylate) Cibacron Blue F3GA Elution with 1 M KSCN [72]
   Magnetic chitosan beads   Magnetically stabilized fluidized bed [73]
  Ornithodoros moubata Magnetic chitin   Elution with alkaline, high salt buffer [74]
  Commercial preparation Magnetic cross-linked polyvinylalcohol Cibacron blue 3GA Elution with high salt buffer [52]
   Magnetite – polyacrylic acid nanoparticles   Ion-exchange separation [75]
   Magnetic cross-linked polyvinylalcohol beads   Adsorption study [76]
  Commercial preparation Magnetic agarose beads Cibacron blue 3GA Magnetically stabilized fluidized bed [77]
   Magnetic chitosan Cibacron blue 3GA Study of adsorption properties [78]
  Commercial preparation Ferrofluid modified sawdust   Elution with 0.5 M NaCl [79]
  Commercial preparation Nano-sized magnetic particles   Elution with NaH2PO4 and NaSCN [80]
Lysozyme (recombinant, histidine-tailed) T4 BioMag, amine terminated Iminodiacetic acid charged with Cu2+ Elution with low pH buffer [81]