From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified enzyme | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
---|---|---|---|---|---|
Lysozyme | Hen egg white | Magnetic chitin | Â | Elution with 0.01 M HCl | [71] |
 | Hen egg white | Magnetic acetylated chitosan |  | Elution with 0.01 M HCl | [9] |
 | Commercial preparation | Magnetic poly(2-hydroxyethyl methacrylate) | Cibacron Blue F3GA | Elution with 1 M KSCN | [72] |
 |  | Magnetic chitosan beads |  | Magnetically stabilized fluidized bed | [73] |
 | Ornithodoros moubata | Magnetic chitin |  | Elution with alkaline, high salt buffer | [74] |
 | Commercial preparation | Magnetic cross-linked polyvinylalcohol | Cibacron blue 3GA | Elution with high salt buffer | [52] |
 |  | Magnetite – polyacrylic acid nanoparticles |  | Ion-exchange separation | [75] |
 |  | Magnetic cross-linked polyvinylalcohol beads |  | Adsorption study | [76] |
 | Commercial preparation | Magnetic agarose beads | Cibacron blue 3GA | Magnetically stabilized fluidized bed | [77] |
 |  | Magnetic chitosan | Cibacron blue 3GA | Study of adsorption properties | [78] |
 | Commercial preparation | Ferrofluid modified sawdust |  | Elution with 0.5 M NaCl | [79] |
 | Commercial preparation | Nano-sized magnetic particles |  | Elution with NaH2PO4 and NaSCN | [80] |
Lysozyme (recombinant, histidine-tailed) | T4 | BioMag, amine terminated | Iminodiacetic acid charged with Cu2+ | Elution with low pH buffer | [81] |