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Table 1 Examples of proteinases and peptidases purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme Source Magnetic carrier Affinity ligand Further details Reference
Aminopeptidase Arabidopsis Amine-terminated magnetic beads N-1-Naphthylphthalamic acid KCl gradient elution [54]
Angiotensin-converting enzyme Pig lung membranes Dynabeads Polyclonal antibodies   [57]
Bromelain Commercial preparation Polyacrylic acid – iron oxide magnetic nanoparticles   Elution with KCl solution [59]
Caspase (recombinant, histidine-tailed) Human cells Magnetic agarose Ni-NTA Elution with SDS-PAGE buffer [60]
Chymotrypsin Commercial preparation Magnetic chitosan beads   Elution with N-acetyl-D-tryptophan [62]
NIa-protease (recombinant, histidine-tagged) Plum Pox Virus Magnetic core and nickel-silica composite matrix Ni2+ Elution with imidazole containing buffer [36]
Proteinases Commercial sources Magnetic cross-linked erythrocytes   Elution with low pH buffer [46]
Proteinase, bacterial (Savinase) Bacillus clausii Silanized magnetite particles Bacitracin   [84]
Trypsin Porcine pancreatin Silanized magnetite particles p-Aminobenzamidine Elution with low pH buffer [50]
  Porcine pancreatin Magnetic polymer particles Soybean trypsin inhibitor Elution with low pH solution [86]
  Commercial preparation Silanized ferrite powder Soybean trypsin inhibitor   [87]
  Commercial preparation Magnetic κ-carrageenan particles Soybean trypsin inhibitor Elution with low pH solution, MSFB [88][89]
  Commercial preparation Magnetic polyacrylamide beads Soybean trypsin inhibitor Magnetically stabilized fluidized beds [90]
  Commercial preparation Magnetic chitosan particles Aprotinin Elution with low pH solution [91]
  Commercial preparation Magnetic cross-linked erythrocytes   Elution with low pH buffer; separation from large volume sample [19]
Urokinase Crude urokinase preparation Magnetic dextran, agarose, polyvinyl alcohol, polyhydroxyethyl methacrylate microspheres p-Aminobenzamide, L-arginine methyl ester, guanidine hexanoic acid or guanidine acetic acid   [93]