Magnetic techniques for the isolation and purification of proteins and peptides

Safarik, Ivo; Safarikova, Mirka

doi:10.1186/1477-044X-2-7

BioMagnetic Research and Technology

Table 1 Examples of proteinases and peptidases purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified enzyme	Source	Magnetic carrier	Affinity ligand	Further details	Reference
Aminopeptidase	Arabidopsis	Amine-terminated magnetic beads	N-1-Naphthylphthalamic acid	KCl gradient elution	[54]
Angiotensin-converting enzyme	Pig lung membranes	Dynabeads	Polyclonal antibodies		[57]
Bromelain	Commercial preparation	Polyacrylic acid – iron oxide magnetic nanoparticles		Elution with KCl solution	[59]
Caspase (recombinant, histidine-tailed)	Human cells	Magnetic agarose	Ni-NTA	Elution with SDS-PAGE buffer	[60]
Chymotrypsin	Commercial preparation	Magnetic chitosan beads		Elution with N-acetyl-D-tryptophan	[62]
NIa-protease (recombinant, histidine-tagged)	Plum Pox Virus	Magnetic core and nickel-silica composite matrix	Ni²⁺	Elution with imidazole containing buffer	[36]
Proteinases	Commercial sources	Magnetic cross-linked erythrocytes		Elution with low pH buffer	[46]
Proteinase, bacterial (Savinase)	Bacillus clausii	Silanized magnetite particles	Bacitracin		[84]
Trypsin	Porcine pancreatin	Silanized magnetite particles	p-Aminobenzamidine	Elution with low pH buffer	[50]
	Porcine pancreatin	Magnetic polymer particles	Soybean trypsin inhibitor	Elution with low pH solution	[86]
	Commercial preparation	Silanized ferrite powder	Soybean trypsin inhibitor		[87]
	Commercial preparation	Magnetic κ-carrageenan particles	Soybean trypsin inhibitor	Elution with low pH solution, MSFB	[88][89]
	Commercial preparation	Magnetic polyacrylamide beads	Soybean trypsin inhibitor	Magnetically stabilized fluidized beds	[90]
	Commercial preparation	Magnetic chitosan particles	Aprotinin	Elution with low pH solution	[91]
	Commercial preparation	Magnetic cross-linked erythrocytes		Elution with low pH buffer; separation from large volume sample	[19]
Urokinase	Crude urokinase preparation	Magnetic dextran, agarose, polyvinyl alcohol, polyhydroxyethyl methacrylate microspheres	p-Aminobenzamide, L-arginine methyl ester, guanidine hexanoic acid or guanidine acetic acid		[93]

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