From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified peptide | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
---|---|---|---|---|---|
Biotinylated peptides | Model mixtures | Dynabeads M-280 streptavidin | Streptavidin | Used in MALDI-TOF mass analysis | [159] |
(His)6-Ala-Tyr-Gly | Synthetic peptide | Dynabeads M-280 tosylactivated | Aminocaproic nitrilotriacetic acid charged with Ni2+ | Elution with imidazole solution | [160] |
Synthetic pentapeptides against fructose-1,6-biphosphate aldolase | Synthetic mixture | Streptavidin-coated magnetic beads | Biotin labelled fructose-1,6-biphosphate aldolase of T. brucei | Pentapeptides were anchored on polystyrene beads | [161] |
Tryptic digest products of cytochrome c | Trypsin digested cytochrome c | Au@magnetic particles | - | Ion-exchange separation followed by MALDI MS analysis | [31] |
Glutathione | Â | Gold and iron oxide nanocomposites | Â | Â | [162] |
Nisin Z | Lactobacillus lactis | EDC activated magnetic beads | Anti-nisin antibody | Elution with 6 M urea | [163] |