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Table 9 Examples of peptides purified by magnetic techniques

From: Magnetic techniques for the isolation and purification of proteins and peptides

Purified peptide

Source

Magnetic carrier

Affinity ligand

Further details

Reference

Biotinylated peptides

Model mixtures

Dynabeads M-280 streptavidin

Streptavidin

Used in MALDI-TOF mass analysis

[159]

(His)6-Ala-Tyr-Gly

Synthetic peptide

Dynabeads M-280 tosylactivated

Aminocaproic nitrilotriacetic acid charged with Ni2+

Elution with imidazole solution

[160]

Synthetic pentapeptides against fructose-1,6-biphosphate aldolase

Synthetic mixture

Streptavidin-coated magnetic beads

Biotin labelled fructose-1,6-biphosphate aldolase of T. brucei

Pentapeptides were anchored on polystyrene beads

[161]

Tryptic digest products of cytochrome c

Trypsin digested cytochrome c

Au@magnetic particles

-

Ion-exchange separation followed by MALDI MS analysis

[31]

Glutathione

 

Gold and iron oxide nanocomposites

  

[162]

Nisin Z

Lactobacillus lactis

EDC activated magnetic beads

Anti-nisin antibody

Elution with 6 M urea

[163]