Table 5 Examples of antibodies purified by magnetic techniques
From: Magnetic techniques for the isolation and purification of proteins and peptides
Purified antibody | Source | Magnetic carrier | Affinity ligand | Further details | Reference |
|---|---|---|---|---|---|
Anti-BODIPY-fluorescein antibodies | Â | Magnetoliposomes | BODIPY-fluorescein | Â | [94] |
Anti-DNA antibody | Systemic lupus erythematosus patient plasma | Magnetic poly(2-hydroxyethyl-methacrylate) beads | DNA | Desorption with 1 M NaSCN solution | [95] |
Anti-human chorionic gonadotropin antibody | Murine ascites supernatants | Magnetic cellulose beads | Human chorionic gonadotropin | Â | [96] |
Antibody (from rat) | Sample from affinity chromatography | Dynabeads M-280 | Sheep anti-rabbit IgG | Antibody concentration | [3] |
Antibody | Rabbit serum | Dynabeads M-280 | Sheep anti-rabbit IgG | Elution with 0.5 M acetic acid | [97] |
Monoclonal antibodies | Mouse hybridoma culture broth | Magnetite particles | Protein A | Â | [98] |
Anti-bovine serum albumin antibodies | Â | Thermosensitive magnetic microspheres | Bovine serum albumin | Immobilization by the carbodiimide method | [99] |
Immunoglobulin G, human | Commercial preparation | Magnetic poly(ethylene glycol dimethacrylate-N-methacryloly-L-histidine-methylester) beads | Â | Elution with 1 M NaCl | [100] |
Immunoglobulin G | Blood serum | Carboxyl-terminated magnetic particles | MproteinAG | Â | [101] |
IgE antibodies | Allergic patients sera | Magnetoliposomes | Antigenic proteins | Â | [16] |
Murine anti-fibroblast growth factor receptor 1 IgM | Ascites | Polystyrene magnetic beads | Rat anti-mouse IgM monoclonal antibody | Â | [102] |